Sampling

MEVAC technical team perform sampling collecting services and analysis with different procedures on regular basis for the following purpose:

1-Monitoring the immune response in the flocks to design expected protection curve for poultry, livestock and fish farms to support customers to design and monitor the vaccination programs and flocks’ health status

2-Monitor the prevalence and occurrence of the different poultry, livestock and fish pathogens and conduct epidemiological surveillance to the different pathogen and in-tern conduct an epidemiological mapping to the different pathogen in the region

3-The immune status and epidemiological mapping services can help the farmer to optimize the animal products and profits and support the governmental efforts to support the poultry and livestock production, in addition to support the governmental strategies to control and eradication Veterinary pathogen.

MEVAC has highly qualified technical team and cutting edges technologies in veterinary problem diagnosis strategies including sample collection in proper manner, pathogen detection and isolation and identification producers either conventional ones or using molecular biology techniques and pathogen genome sequencing and genotyping.

- PCR
- HI
- ELISA
- Sequencing

HI- HA assays: Hemagglutinating viruses like Influenza virus particles have an envelope protein called the hemagglutinin, or HA, which binds to sialic acid receptors on cells. The virus will also bind to erythrocytes (red blood cells), causing the formation of a lattice. This property is called hemagglutination and is the basis of a rapid assay to determine levels of influenza virus present in a sample. To conduct the assay, two-fold serial dilutions of a virus are prepared, mixed with a specific amount of red blood cells, and added to the wells of a plastic tray. The red blood cells that are not bound by influenza virus sink to the bottom of a well and form a button. The red blood cells that are attached to virus particles form a lattice that coats the well. The assay can be performed within 30 minutes and is therefore a quick indicator of the relative quantities of virus particles

HI- HA assays: Hemagglutinating viruses like Influenza virus particles have an envelope protein called the hemagglutinin, or HA, which binds to sialic acid receptors on cells. The virus will also bind to erythrocytes (red blood cells), causing the formation of a lattice. This property is called hemagglutination and is the basis of a rapid assay to determine levels of influenza virus present in a sample. To conduct the assay, two-fold serial dilutions of a virus are prepared, mixed with a specific amount of red blood cells, and added to the wells of a plastic tray. The red blood cells that are not bound by influenza virus sink to the bottom of a well and form a button. The red blood cells that are attached to virus particles form a lattice that coats the well. The assay can be performed within 30 minutes and is therefore a quick indicator of the relative quantities of virus particles

The Molecular Biology section utilizes Polymerase Chain Reaction (PCR) technology to detect the presence of nucleic acids (DNA/RNA) from pathogenic organisms in clinical samples. Molecular diagnostic tests are fast and more sensitive when compared to other conventional methods such as bacterial culture or virus isolation. A wide variety of tests are available for bacteria, fungi, and viruses from many animal species, including pathogens of Poultry, cattle, cats, dogs, horses, marine mammals and laboratory animals. The techniques can make the discrimination of non-pathogenic from pathogenic strains by detection of specific genes in several pathogen types.

Sequencing the genetic material (DNA or RNA) of infectious agents can be a powerful tool for identifying an agent, for monitoring the changes in a disease agent and for the discovery of new or emerging diseases ME VAC Molecular Biology section has several agreements with different institutes and organization to conduct sequencing procedures either conventional sequencing or next generation sequencing and implanted it in diagnosis, problem solving and research purposes

The ELISA (enzyme-linked immunosorbent assay) is one of the most sensitive and reproducible diagnostic technologies available. These assays are rapid, simple to perform and easily automated. As with any assay, the reproducibility and reliability of ELISAs are dependent upon proper technique and attention to detail. MEVAV facility have serological section which run variety of ELISA kits for the quantitative detection of veterinary infections, zoonotic pathogens as well as veterinary drugs that facilitate the research or clinical treatment of veterinary science.